Reduction of soybean nodulation with standard inoculation associated with Bacillus subtilis on farm
Keywords:
bioinputs, microorganisms, soil healthAbstract
Current agriculture faces the challenge of reconciling productivity with environmental preservation and public health. One Health, which integrates human, animal and environmental aspects, highlights the importance of soil health for the sustainability of agricultural systems. In this context, the use of bioinputs produced “on farm” represents a potential risk, due to the lack of quality control and the possibility of microbiological contamination. This study aimed to evaluate the effects of standard inoculation and standard inoculation associated with the use of Bacillus subtilis multiplied “on farm” in soybean crops. The experiment was conducted in a greenhouse, with a completely randomized design, two treatments and six replicates. At 40 days after emergence, statistically significant differences were observed for the number of nodules, fresh nodule mass and dry nodule mass. The treatment with standard inoculation showed an average of 10.66 nodules, 28.53 mg of fresh nodule mass and 9.93 mg of dry nodule mass, while the treatment with standard inoculation plus the “on farm” product reduced these values to 4 nodules, 9.97 mg of MNF and 3.52 mg of MNS. The results indicate that the use of “on farm” inoculants compromises the efficacy of nodulation in soybean, which can negatively affect the productivity and sustainability of the system. It is concluded that the use of these products should be carried out with caution, considering the risks associated with microbiological quality.
References
BOCATTI, C. R. et al. Microbiological quality analysis of inoculants based on Bradyrhizobium spp. and Azospirillum brasilense produced “on farm” reveals high contamination with non-target microorganisms. Brazilian Journal of Microbiology, v. 53, n. 1, p. 267-280, 2022.
FERREIRA, E. B. et al. ExpDes: an R package for ANOVA and experimental designs. Applied Mathematics, v. 5, n. 19, p. 2952, 2014.
HUNGRIA, M. et al. Inoculum rate effects on the soybean symbiosis in new or old fields under tropical conditions. Agronomy Journal, v. 109, p. 1106-1112, 2017. DOI: 10.2134/agronj2016.11.0641
KOVALSKI, E. et al. Resultados preliminares da produção de inoculantes em fazenda na região de Curitibanos-SC. Revista Latinoamericana Ambiente e Saúde, v. 3 (especial), p. 268-275, 2023.
KOVALSKI, E. et al. Análises microbiológicas de biosumos multiplicados a partir de Bacillus e Trichoderma em biofábricas na região de Curitibanos–SC. Saúde e Meio Ambiente: Revista Interdisciplinar, v. 131-149, 2024b.
KOVALSKI, E. et al. Avaliação da qualidade microbiológica de Trichoderma asperellum produzido em sistema on farm em Curitibanos–SC. 2024a.
LANA, U. G. P. et al. Avaliação da qualidade de biopesticidas à base de Bacillus thuringiensis produzidos em sistema" on farm". 2019.
LANA, U. G. P. et al. Avaliação da qualidade de inoculates à base de Bacillus para promoção de crescimento de plantas produzidos em sistema on farm. 2022.
SANTOS, A.; DINNAS, S.; FEITOZA, A. Qualidade microbiológica de bioprodutos comerciais multiplicados on farm no Vale do São Francisco: dados preliminares. Enciclopédia Biosfera, v. 17, n. 34, p. 01-15, 2020.
VALICENTE, F. H. et al. Riscos à produção de biopesticida à base de Bacillus thuringiensis. Circular Técnica, v. 239, p. 20, 2018.
